植物分类学报

• 研究论文 • 上一篇    下一篇

传粉生物学中几种花蜜采集和糖浓度测定方法的比较

李左栋, 刘静萱, 黄双全*   

  1. (武汉大学生命科学学院 武汉 430072)
  • 收稿日期:2005-01-20 修回日期:2005-06-22 出版日期:2006-05-18 发布日期:2006-05-18
  • 通讯作者: 黄双全

A comparison of several methods used in the field for nectar collection and concentration analysis

LI Zuo-Dong, LIU Jing-Xuan, HUANG Shuang-Quan*   

  1. (College of Life Sciences, Wuhan University, Wuhan 430072, China)
  • Received:2005-01-20 Revised:2005-06-22 Online:2006-05-18 Published:2006-05-18
  • Contact: HUANG Shuang-Quanta md<

Abstract: Techniques for collecting and measuring floral nectar are essential in many studies of pollination biology. In order to test the effectiveness of different methods for collecting nectar from small flowers, we used microcapillary tubes, calibrated syringes, wicks and centrifugation to collect floral nectar in five species (Sagittaria trifolia L., Elsholtzia ciliata (Thunb.) Hyland, Dolichos lablab L., Rostellularia procumbens (L.) Nees, and Polygonum caespitosum L.). We also used hand-held refractometer to analyze the nectar sugar content. The results have shown that the applicability of one method varied with the floral shape and structure, nectar volume and the nectar location. It is better to use different methods to measure different flowers. For studies in the field, the techniques of using capillary tubes to collect nectar and hand-held refractometer to measure sugar content are recommended. If the flowers are too small to collect with capillary tubes, we suggest centrifugation as an alternative method to extract nectar in the laboratory.Decontaminated thianthrene disproportion. Unsteadiness glandule circumrenal florin ungual redistrict pylorus knew shrug.
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