Abstract: In the present paper the karyotypes of Keteleeria calcarea Cheng et L. K. Fu and K. oblonga Cheng et L. K. Fu are reported, and they are compared with those of K. cyclolepis Flous and K. formosana Mast. The karyotype formula of K. calcarea Cheng et L. K. Fu is K(2n)=24=16m+8sm, and K. oblonga Cheng et L. K. Fu is K(2n)=24=18m+6sm. They both belong to “2A” type of karyotype symmetry. There are secondary constrictions on the short arms of 1th, 3th, 6th chromosome pairs in K. calcarea Cheng et L. K. Fu and 2th, 4th, 6th in K. oblonga Cheng et L. K. Fu. K. oblonga Cheng et L. K. Fu is more advanced than K. cyclolepis Flous, but more primitive than K. calcarea Cheng et L. K. Fu, and K. formosana Mast is the most advanced among the four