植物分类学报

• 研究论文 • 上一篇    下一篇

茶树细胞分类学研究

梁国鲁, 林蒙嘉, 陈家玉, 刘君素   

  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1992-11-10 发布日期:1992-11-10
  • 通讯作者: 梁国鲁

Cytotaxonomical Studies of Tea Plants

Liang Guo-Lu, Lin Meng-Jia, Chen Jia-Yu, Liu Jun-Su   

  • Received:1900-01-01 Revised:1900-01-01 Online:1992-11-10 Published:1992-11-10
  • Contact: Liang Guo-Lu

Abstract: Nine forms of Camellia sinensis (L) Kuntze from Guizhou, Yunnan, Sichuan and Fujian Provinces were cytotaxonomically studied in this work. The wall degradation hypotonic method was used for preparing chromosome samples which were stained in Giemsa. The micrographs of their somatic metaphase are shown in Plates 1-2, the ranges of chromosome relative lengths, arm ratios, karyotype  formulae  and  classification  of karyotypes  according  to  Li  and Chen (1985) are shown in Table 1 and the idiograms in Fig. l. The main points are mentioned as follows:   (1) All these forms are found to be diploid with 2n = 30, and they are reported for the first time except Yunnan(Fengqing)cultivated form.   (2) All the karyotypes of the forms examined are relatively uniform, which re- veal the gradual decrease in size from the longest to the shortest chromosomes, similar chromosomal relative length, smaller chromosome types varying between 2- 5μm. The karyotype mainly consists  of metacentric (m)  and  submeta- centric (sm) chromosomes.  Sat-chromosomes  are difficult  to be discovered on mid -metaphase chromosomes. The cytological information confirms the close relationship among these tea forms.   (3) According to the asymmetry of karyotype, the nine tea forms are divided into two types: one consists of the arbor with macrophyll, i.e. Guizhou and Yunnan( Fengqing and Tengchong)cultivated forms,  which have the”2A” karyotype possessing metacentric(m) and submetacentric(sm) chromosomes. The karyotypes might be of the most symmetrical or primitive type ;the other consists of the bush or  microarbor with mostly medium- and microphyll, less frequently macrophyll, i.e. Qianmei 419, Qianmei 502, Yaan,Fuding, Sichuan and Qianmei 101 cultivated forms, which have the “2A” and “2B” karyotypes, consisting of metacentric (m),  submetacentric (sm)  and  a  pair  of subtelocentric  (st) chromosomes, and more asymmetrical than that of the first karyotype.  They might be more advanced.   (4) In recent years, two subspecies, i.e. ssp. “yunnan”and ssp. “bohea”, including seven varieties, in C. sinensis were recognized but not formally published by Zhuang et al. (1981). From the present work and the previous reports the au- thors agree with Zhuang’s classification, because recognition of two subspecies is re- markably consistent with the two kinds of karyotypes reported here. C.sinensis ssp. “yunnan” with arbor and macrophyll characters has more symmetrical karyotype than that of C. sinensis ssp. “bohea”  with bush or microarbor and mostly medium- and microphyll characters. That is to say, ssp.”yunnan” is more primitive than ssp. “bohea”. (5) Based on the karyotyp

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