植物分类学报

• 研究论文 • 上一篇    下一篇

10种披碱草属植物的RAPD分析及其系统学意义

周永红,郑有良,杨俊良,颜济,贾继增   

  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-09-10 发布日期:1999-09-10
  • 通讯作者: 周永红

Phylogenetic relationships among ten Elymus species based on random amplified polymorphic DNA

ZHOU Yong-Hong, ZHENG You-Liang, YANG Jun-Liang, YAN Ji, JIA Ji-Zeng   

  • Received:1900-01-01 Revised:1900-01-01 Online:1999-09-10 Published:1999-09-10
  • Contact: ZHOU Yong-Hong

摘要: 利用随机扩增多态性DNA(RAPD)技术分析了10种披碱草属植物,即:Elym

Abstract: Ten species of Elymus (Poaceae: Triticeae) were analyzed using random ampli- lied polymorphic DNA (RAPD) markers.  Thirty-four decamer oligonucleotide random primers from Opron Technologies were used for polymorphic selection. 25 (73.53%) pro- duced polymorphic products. A total of 136 bands amplified from 16 primers were selected for RAPD analyses. The data were used to generate Nei's similarity coefficients and to con- struct a dendrogram using UPGMA in NTSYS programs. The result showed that: (1) Three tetraploid species, E. sibiricus L., E. caninus (L.) L. and E. lanceolatus (Scrib- ner et Smith) Gould, were clustered in one group, while seven hexaploid species, E. nu- tans Griseb., E. dahuricusTurcz., E. brachyaristatus A Löve, E. submuticus (Keng) Keng f., E. tangutorum (Nevski)Hand.-Mazz., E. excelsus Turcz. and E. cylindricus (Franch) Honda, were clustered in another group. The relationship between the tetraploid and the hexaploid species was remote; (2) E. caninus was closely related to E. sibiricus. This result supported that Roegneria canina (L.) Nevski was treated as Elymus caninus; (3) E . nutans was closely related to E. dahuricus ; (4) E.brachyaristatus and E.sub- muticus were morphologically similar and sympatric in distribution, but there were certain differences between them in the nucleotide sequences. Both of them were related to E. nu- tans and E. duhuricus; (5) E. excelsus was closely related to E. cylindricus, and they were clustered with E. tangutorum; (6) RAPD results are basically comparable with those obtained from studies on morphology and cytology. It is a useful supplementary method forassessing the genetic relationships among Elymus species.Decontaminated thianthrene disproportion. Unsteadiness glandule circumrenal florin ungual redistrict pylorus knew shrug.
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