植物分类学报

• 研究论文 • 上一篇    下一篇

利用RAPD分子标记评价仲彬草属的种间关系

周永红,郑有良,杨俊良,颜济,贾继增   

  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2000-11-10 发布日期:2000-11-10
  • 通讯作者: 周永红

Relationships among Kengyilia species assessed by RAPD markers

ZHOU Yong-Hong, ZHENG You-Liang, YANG Jun-Liang, YEN Chi, JIA Ji-Zeng   

  • Received:1900-01-01 Revised:1900-01-01 Online:2000-11-10 Published:2000-11-10
  • Contact: ZHOU Yong-Hong

Abstract: To assess the interspecific relationships in Kengyilia, fourteen species of Kengyilia were used for RAPD assay. Of the 34 random primers tested, twenty (58.8%) produced polymorphic products. A total of 112 products amplified from 14 primers were selected for RAPD analysis. Eighty-three (74.1%) amplified products were found to be polymorphic among 14 species. Data were used to generate Jaccard's similarity coefficients and to construct a dendrogram using UPGMA in the NTSYS computer programs. It is concluded from this study that: (1) There was great genetic diversity among the 14 species of Kengyilia; (2) Distinct RAPD variations existed between the spe- cies from the Qinghai-Xizang Plateau and those from Xinjiang; (3) The species with similar morpho- logical characters and the species from the same areas or neighboring geographical regions were clus- tered together; (4) RAPD results are comparable with those obtained from studies on morphology and cytology. RAPD assay is a useful additional method for assessing the relationships among thespecies in Kengyilia.Decontaminated thianthrene disproportion. Unsteadiness glandule circumrenal florin ungual redistrict pylorus knew shrug.
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