植物分类学报

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利用分子标记技术对鲜切花“黄莺花”的鉴定和分类

马玲; 强胜   

  1. (南京农业大学杂草研究室 南京 210095)
  • 收稿日期:2006-08-28 修回日期:2006-12-04 出版日期:2007-07-18 发布日期:2007-07-18
  • 通讯作者: 强胜hd E-mail:wrl@njau.edu.cn, qiangs@njau.edu.cncom

Identification and classification of cut-flower “Huangyinghua”using DNA marker techniques

MA Ling, QIANG Sheng*   

  1. (Weed Research Laboratory, Nanjing Agricultural University, Nanjing 210095, China)
  • Received:2006-08-28 Revised:2006-12-04 Online:2007-07-18 Published:2007-07-18
  • Contact: QIANG Sheng E-mail:wrl@njau.edu.cn, qiangs@njau.edu.cncom

Abstract: rch Laboratory, Nanjing Agricultural University, Nanjing 210095, China)
Abstract “Huangyinghua” is a popular cut-flower in China, but it is unclear as to whether
“Huangyinghua” is an invasive Solidago canadensis or not. The genetic relationship of a total
of 45 samples of “Huangyinghua” with S. canadensis, and S. decurrens were investigated
using AFLP technique so as to determine the identity of “Huangyinghua”. Genomic DNA was
digested with EcoRI and MseI enzymes and amplified with six E+3 and M+3 primer
combinations. AFLP analysis produced 661 endonucleotide discernable bands, of which 639
(96.61%) were polymorphic. Cluster analysis through using UPGMA method indicated that
“Huangyinghua” and S. canadensis were clustered into the same group that was different
from S. decurrens. Sequence analysis based on the ITS regions showed that their sequences of
5.8S rDNA were the same, and the differences were found only in the ITS1 and ITS2 regions.
ITS phylogenetic trees of the tested samples and closely-related species were reconstructed
based on our sequence data in combination with those from GenBank. Based on the trees,
“Huangyinghua” was found to belong to S. canadensis complex, but not to S. decurrens.
Moreover, it was found that there were considerable genetic variation in both
“Huangyinghua” and S. canadensis. Therefore, the cut-flower “Huangyinghua” may be
invasive, and proper measures should be taken to control the further spread of its propagules.

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