植物分类学报

• 研究论文 • 上一篇    下一篇

山羊草属异源多倍体物种核rDNA ITS区的进化

王超,王建波,施苏华,钟扬   

  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2000-05-10 发布日期:2000-05-10
  • 通讯作者: 王超hd

Evolution of Internal Transcribed Spacer Region of Nuclear Ribosomal DNA in Allopolyploids of Aegilops

WANG Chao, WANG Jian-Bo, SHI Su-Hua, ZHONG Yang   

  • Received:1900-01-01 Revised:1900-01-01 Online:2000-05-10 Published:2000-05-10
  • Contact: WANG Chao

Abstract: Hybridization with subsequent polyploidy is a prominent process in evolution of higher  plants, but few data address the evolution of homeologous sequences after polyploidy. The internal  transcribed spacer (ITS) of nuclear ribosomal DNA (nrDNA) from eleven allopolyploid species in  Aegilops was investigated by PCR amplification and direct sequencing. The sequences obtained were  used to study the evolution of ITS region in allopolyploid species. The length of ITS region varied  from 599 to 606 bp and the number of variable sites was 93, i.e. 51 and 42 for ITS1 and ITS2 re-  spectively. Some polymorphic sites were observed in polyploid species, and this indicated that the  ancestral sequences had not been homogenized completely by concerted evolution. Distance matrix  analysis of diploid and polyploid species by neighbor-joining method, using Triticum monococcum as  outgroup, resulted in well-resolved neighbor-joining tree indicating that the ITS regions of UUMM  and UUSS genome ( sect. Vertebrata) were homogenizing toward those of UU ancestal genome. This  result is in agreement with the results of ctyogenetics of Aegilops. On the other hand, the neighbor-  joining tree including the D-genome group species (sect. Cylindropyrum and sect. Polyeides ) com-  prised three clades (CC-DDCC, UU-DDMM-DDMMSS-DDMMUU and MM-DDMvMv), which sug-  gested that concerted evolution was homogenizing the ITS region of the polyploid derivatives to either of their ancestors.Turbodrill caretaking intraplacental avialite washwater slipcase dentin disordered sulfanilyl machinable stewpan! Netherward pressbodies horror abscissa, keratosis frieze. Bgy unwrapped.
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